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human keap1 overexpression clone lentiviral particle rc202189l4v  (OriGene)


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    OriGene human keap1 overexpression clone lentiviral particle rc202189l4v
    Activation of Nrf2 and Nrf2-dependent HIF1α expression in the cells treated by iAs. A. Time-dependent activation of Nrf2 and HIF1α. B. Involvement of JNK in iAs-induced Nrf2 as well as HIF1α. C. Luciferase reporter gene activities of Nrf2 and HIF1α in the cells treated with 1 μM iAs for the indicated times. D. <t>Lentiviral</t> transfection of Keap1 inhibited iAs-induced Nrf2 activation and HIF1α expression. E. Knockout of Nrf2 by CRISPR-Cas9 gene editing prevented HIF1α induction by iAs. Red asterisk denotes the non-specific (N.S.) band.
    Human Keap1 Overexpression Clone Lentiviral Particle Rc202189l4v, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+keap1+overexpression/monoclonal+anti+keap1/pmc07086359-52-4-15
    Average 90 stars, based on 1 article reviews
    human keap1 overexpression clone lentiviral particle rc202189l4v - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "Nrf2 and HIF1α converge to arsenic-induced metabolic reprogramming and the formation of the cancer stem-like cells"

    Article Title: Nrf2 and HIF1α converge to arsenic-induced metabolic reprogramming and the formation of the cancer stem-like cells

    Journal: Theranostics

    doi: 10.7150/thno.42903

    Activation of Nrf2 and Nrf2-dependent HIF1α expression in the cells treated by iAs. A. Time-dependent activation of Nrf2 and HIF1α. B. Involvement of JNK in iAs-induced Nrf2 as well as HIF1α. C. Luciferase reporter gene activities of Nrf2 and HIF1α in the cells treated with 1 μM iAs for the indicated times. D. Lentiviral transfection of Keap1 inhibited iAs-induced Nrf2 activation and HIF1α expression. E. Knockout of Nrf2 by CRISPR-Cas9 gene editing prevented HIF1α induction by iAs. Red asterisk denotes the non-specific (N.S.) band.
    Figure Legend Snippet: Activation of Nrf2 and Nrf2-dependent HIF1α expression in the cells treated by iAs. A. Time-dependent activation of Nrf2 and HIF1α. B. Involvement of JNK in iAs-induced Nrf2 as well as HIF1α. C. Luciferase reporter gene activities of Nrf2 and HIF1α in the cells treated with 1 μM iAs for the indicated times. D. Lentiviral transfection of Keap1 inhibited iAs-induced Nrf2 activation and HIF1α expression. E. Knockout of Nrf2 by CRISPR-Cas9 gene editing prevented HIF1α induction by iAs. Red asterisk denotes the non-specific (N.S.) band.

    Techniques Used: Activation Assay, Expressing, Luciferase, Transfection, Knock-Out, CRISPR

    iAs induces expression of a number of stemness genes through Nrf2 and/or HIF1α activation. A. Enrichment status of Nrf2 and HIF1α (pointed by red arrows) induced by iAs on these indicated stemness genes as determined by ChIP-seq. B. De novo and/or known Nrf2 motif(s) induced by iAs on the promoter or gene body of MYC, CD44, KLF4, and EGFR that had been linked to the stemness of CSCs. C. Nrf2 inhibition by lentiviral Keap1 transfection prevented induction of MYC and SOX2 by iAs. This Nrf2 inhibition also reduced KLF4 expression. D. Nrf2 knockout blocked iAs-induced expression of MYC, SOX2, BACH1, TBC1D7, and decreased expression of KLF4.
    Figure Legend Snippet: iAs induces expression of a number of stemness genes through Nrf2 and/or HIF1α activation. A. Enrichment status of Nrf2 and HIF1α (pointed by red arrows) induced by iAs on these indicated stemness genes as determined by ChIP-seq. B. De novo and/or known Nrf2 motif(s) induced by iAs on the promoter or gene body of MYC, CD44, KLF4, and EGFR that had been linked to the stemness of CSCs. C. Nrf2 inhibition by lentiviral Keap1 transfection prevented induction of MYC and SOX2 by iAs. This Nrf2 inhibition also reduced KLF4 expression. D. Nrf2 knockout blocked iAs-induced expression of MYC, SOX2, BACH1, TBC1D7, and decreased expression of KLF4.

    Techniques Used: Expressing, Activation Assay, ChIP-sequencing, Inhibition, Transfection, Knock-Out



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    human keap1 overexpression clone lentiviral particle rc202189l4v  (OriGene)
    90
    OriGene human keap1 overexpression clone lentiviral particle rc202189l4v
    Activation of Nrf2 and Nrf2-dependent HIF1α expression in the cells treated by iAs. A. Time-dependent activation of Nrf2 and HIF1α. B. Involvement of JNK in iAs-induced Nrf2 as well as HIF1α. C. Luciferase reporter gene activities of Nrf2 and HIF1α in the cells treated with 1 μM iAs for the indicated times. D. <t>Lentiviral</t> transfection of Keap1 inhibited iAs-induced Nrf2 activation and HIF1α expression. E. Knockout of Nrf2 by CRISPR-Cas9 gene editing prevented HIF1α induction by iAs. Red asterisk denotes the non-specific (N.S.) band.
    Human Keap1 Overexpression Clone Lentiviral Particle Rc202189l4v, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+keap1+overexpression/monoclonal+anti+keap1/pmc07086359-52-4-15
    Average 90 stars, based on 1 article reviews
    human keap1 overexpression clone lentiviral particle rc202189l4v - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier
    human keap1 overexpression  (OriGene)
    90
    OriGene human keap1 overexpression
    Activation of Nrf2 and Nrf2-dependent HIF1α expression in the cells treated by iAs. A. Time-dependent activation of Nrf2 and HIF1α. B. Involvement of JNK in iAs-induced Nrf2 as well as HIF1α. C. Luciferase reporter gene activities of Nrf2 and HIF1α in the cells treated with 1 μM iAs for the indicated times. D. Lentiviral transfection of <t>Keap1</t> inhibited iAs-induced Nrf2 activation and HIF1α expression. E. Knockout of Nrf2 by CRISPR-Cas9 gene editing prevented HIF1α induction by iAs. Red asterisk denotes the non-specific (N.S.) band.
    Human Keap1 Overexpression, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+keap1+overexpression/KEAP1+(NM_203500)+Human+Tagged+ORF+Clone+Lentiviral+Particle/pmc07086359-52-0-15
    Average 90 stars, based on 1 article reviews
    human keap1 overexpression - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Activation of Nrf2 and Nrf2-dependent HIF1α expression in the cells treated by iAs. A. Time-dependent activation of Nrf2 and HIF1α. B. Involvement of JNK in iAs-induced Nrf2 as well as HIF1α. C. Luciferase reporter gene activities of Nrf2 and HIF1α in the cells treated with 1 μM iAs for the indicated times. D. Lentiviral transfection of Keap1 inhibited iAs-induced Nrf2 activation and HIF1α expression. E. Knockout of Nrf2 by CRISPR-Cas9 gene editing prevented HIF1α induction by iAs. Red asterisk denotes the non-specific (N.S.) band.

    Journal: Theranostics

    Article Title: Nrf2 and HIF1α converge to arsenic-induced metabolic reprogramming and the formation of the cancer stem-like cells

    doi: 10.7150/thno.42903

    Figure Lengend Snippet: Activation of Nrf2 and Nrf2-dependent HIF1α expression in the cells treated by iAs. A. Time-dependent activation of Nrf2 and HIF1α. B. Involvement of JNK in iAs-induced Nrf2 as well as HIF1α. C. Luciferase reporter gene activities of Nrf2 and HIF1α in the cells treated with 1 μM iAs for the indicated times. D. Lentiviral transfection of Keap1 inhibited iAs-induced Nrf2 activation and HIF1α expression. E. Knockout of Nrf2 by CRISPR-Cas9 gene editing prevented HIF1α induction by iAs. Red asterisk denotes the non-specific (N.S.) band.

    Article Snippet: Human Keap1 overexpression clone lentiviral particle (RC202189L4V) and Lentiviral control particle (PS100093V) were purchased from Origene Technologies Inc. BEAS-2B cells were seeded in 24-wells plate (5 × 10 4 cells per well) and incubated for 20 hours, and then infected with Keap1 lentiviral overexpression particle and Lentiviral control particle for 20 hours, respectively.

    Techniques: Activation Assay, Expressing, Luciferase, Transfection, Knock-Out, CRISPR

    iAs induces expression of a number of stemness genes through Nrf2 and/or HIF1α activation. A. Enrichment status of Nrf2 and HIF1α (pointed by red arrows) induced by iAs on these indicated stemness genes as determined by ChIP-seq. B. De novo and/or known Nrf2 motif(s) induced by iAs on the promoter or gene body of MYC, CD44, KLF4, and EGFR that had been linked to the stemness of CSCs. C. Nrf2 inhibition by lentiviral Keap1 transfection prevented induction of MYC and SOX2 by iAs. This Nrf2 inhibition also reduced KLF4 expression. D. Nrf2 knockout blocked iAs-induced expression of MYC, SOX2, BACH1, TBC1D7, and decreased expression of KLF4.

    Journal: Theranostics

    Article Title: Nrf2 and HIF1α converge to arsenic-induced metabolic reprogramming and the formation of the cancer stem-like cells

    doi: 10.7150/thno.42903

    Figure Lengend Snippet: iAs induces expression of a number of stemness genes through Nrf2 and/or HIF1α activation. A. Enrichment status of Nrf2 and HIF1α (pointed by red arrows) induced by iAs on these indicated stemness genes as determined by ChIP-seq. B. De novo and/or known Nrf2 motif(s) induced by iAs on the promoter or gene body of MYC, CD44, KLF4, and EGFR that had been linked to the stemness of CSCs. C. Nrf2 inhibition by lentiviral Keap1 transfection prevented induction of MYC and SOX2 by iAs. This Nrf2 inhibition also reduced KLF4 expression. D. Nrf2 knockout blocked iAs-induced expression of MYC, SOX2, BACH1, TBC1D7, and decreased expression of KLF4.

    Article Snippet: Human Keap1 overexpression clone lentiviral particle (RC202189L4V) and Lentiviral control particle (PS100093V) were purchased from Origene Technologies Inc. BEAS-2B cells were seeded in 24-wells plate (5 × 10 4 cells per well) and incubated for 20 hours, and then infected with Keap1 lentiviral overexpression particle and Lentiviral control particle for 20 hours, respectively.

    Techniques: Expressing, Activation Assay, ChIP-sequencing, Inhibition, Transfection, Knock-Out

    Activation of Nrf2 and Nrf2-dependent HIF1α expression in the cells treated by iAs. A. Time-dependent activation of Nrf2 and HIF1α. B. Involvement of JNK in iAs-induced Nrf2 as well as HIF1α. C. Luciferase reporter gene activities of Nrf2 and HIF1α in the cells treated with 1 μM iAs for the indicated times. D. Lentiviral transfection of Keap1 inhibited iAs-induced Nrf2 activation and HIF1α expression. E. Knockout of Nrf2 by CRISPR-Cas9 gene editing prevented HIF1α induction by iAs. Red asterisk denotes the non-specific (N.S.) band.

    Journal: Theranostics

    Article Title: Nrf2 and HIF1α converge to arsenic-induced metabolic reprogramming and the formation of the cancer stem-like cells

    doi: 10.7150/thno.42903

    Figure Lengend Snippet: Activation of Nrf2 and Nrf2-dependent HIF1α expression in the cells treated by iAs. A. Time-dependent activation of Nrf2 and HIF1α. B. Involvement of JNK in iAs-induced Nrf2 as well as HIF1α. C. Luciferase reporter gene activities of Nrf2 and HIF1α in the cells treated with 1 μM iAs for the indicated times. D. Lentiviral transfection of Keap1 inhibited iAs-induced Nrf2 activation and HIF1α expression. E. Knockout of Nrf2 by CRISPR-Cas9 gene editing prevented HIF1α induction by iAs. Red asterisk denotes the non-specific (N.S.) band.

    Article Snippet: Human Keap1 overexpression clone lentiviral particle (RC202189L4V) and Lentiviral control particle (PS100093V) were purchased from Origene Technologies Inc. BEAS-2B cells were seeded in 24-wells plate (5 × 10 4 cells per well) and incubated for 20 hours, and then infected with Keap1 lentiviral overexpression particle and Lentiviral control particle for 20 hours, respectively.

    Techniques: Activation Assay, Expressing, Luciferase, Transfection, Knock-Out, CRISPR

    iAs induces expression of a number of stemness genes through Nrf2 and/or HIF1α activation. A. Enrichment status of Nrf2 and HIF1α (pointed by red arrows) induced by iAs on these indicated stemness genes as determined by ChIP-seq. B. De novo and/or known Nrf2 motif(s) induced by iAs on the promoter or gene body of MYC, CD44, KLF4, and EGFR that had been linked to the stemness of CSCs. C. Nrf2 inhibition by lentiviral Keap1 transfection prevented induction of MYC and SOX2 by iAs. This Nrf2 inhibition also reduced KLF4 expression. D. Nrf2 knockout blocked iAs-induced expression of MYC, SOX2, BACH1, TBC1D7, and decreased expression of KLF4.

    Journal: Theranostics

    Article Title: Nrf2 and HIF1α converge to arsenic-induced metabolic reprogramming and the formation of the cancer stem-like cells

    doi: 10.7150/thno.42903

    Figure Lengend Snippet: iAs induces expression of a number of stemness genes through Nrf2 and/or HIF1α activation. A. Enrichment status of Nrf2 and HIF1α (pointed by red arrows) induced by iAs on these indicated stemness genes as determined by ChIP-seq. B. De novo and/or known Nrf2 motif(s) induced by iAs on the promoter or gene body of MYC, CD44, KLF4, and EGFR that had been linked to the stemness of CSCs. C. Nrf2 inhibition by lentiviral Keap1 transfection prevented induction of MYC and SOX2 by iAs. This Nrf2 inhibition also reduced KLF4 expression. D. Nrf2 knockout blocked iAs-induced expression of MYC, SOX2, BACH1, TBC1D7, and decreased expression of KLF4.

    Article Snippet: Human Keap1 overexpression clone lentiviral particle (RC202189L4V) and Lentiviral control particle (PS100093V) were purchased from Origene Technologies Inc. BEAS-2B cells were seeded in 24-wells plate (5 × 10 4 cells per well) and incubated for 20 hours, and then infected with Keap1 lentiviral overexpression particle and Lentiviral control particle for 20 hours, respectively.

    Techniques: Expressing, Activation Assay, ChIP-sequencing, Inhibition, Transfection, Knock-Out